Use this Coomassie brilliant blue R-250 solution to stain proteins in SDS-PAGE gels.
- Ready to use for fast and easy staining
- Mixture of water, methanol, and glacial acetic acid
Protocol Overview
Step 1: Stain gels for 1–2 hours with gentle agitation.
Stain overnight or longer if needed.
Step 2: Destain gels for 2 hours.
Change destaining solution (1610438) multiple times (e.g., 4 washes x 30 min) until the background is less dark.
Packaging Options
- 1 L (1610436)
- 4 x 1 L (1610437)
- 5 L (1610787)
Related Products
- Coomassie Brilliant Blue R-250 staining solutions kit (1610435)
- Coomassie Brilliant Blue R-250 powder, 10 g (1610400)
- Coomassie Brilliant Blue G-250 powder, 10 g (1610406)
- Coomassie destaining solution: 1 L (1610438) or 4 x 1 L (1610439)
Item #: FB2389
Price: $144.08
Ships directly from the manufacturer.
Kit for staining protein-containing polyacrylamide gels, includes 1 L Coomassie Brilliant Blue R-250 staining solution and 2 x 1 L destaining solution; education use only
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This item can only be shipped to schools, museums and science centers
Product Details
Coomassie (Brilliant) Blue R-250 staining solution is the easiest way to fix and detect proteins in polyacrylamide gels. Ready-to-use solutions are packaged in 1 L sizes and eliminate the need to weigh powders or dilute solutions.
Bio-Rad Item No.: 1610435EDU
Specifications
Coomassie (Brilliant) Blue R-250 staining solution is the easiest way to fix and detect proteins in polyacrylamide gels. Ready-to-use solutions are packaged in 1 L sizes and eliminate the need to weigh powders or dilute solutions.
- Richard J. Simpson
This protocol was adapted from “Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins,” Chapter 7, in Proteins and Proteomics by Richard J. Simpson. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.
INTRODUCTION
The most commonly used dye for visualizing proteins in SDS-PAGE gels is Coomassie Brilliant Blue R250 (CBR-250) because of its relatively high sensitivity. This protocol describes the standard CBR-250 staining method, along with a simple method for preparing stained gels for long-term storage.