Thermo Fisher Scientific has an overall rating of 4.0 Average Rating out of 5, based on over 109 Thermo Fisher Scientific Review Ratings left anonymously by Thermo Fisher Scientific employees, which is 3% higher than the average rating for all companies on CareerBliss. 99% of employees would recommend working at Thermo Fisher Scientific.
Does Thermo Fisher Scientific pay their employees well?
Thermo Fisher Scientific employees earn $61,000 annually on average, or $29 per hour, which is 8% lower than the national salary average of $66,000 per year. 64 Thermo Fisher Scientific employees have shared their salaries on CareerBliss. Find Thermo Fisher Scientific Salaries by Job Title.
How satisfied are employees working at Thermo Fisher Scientific?
99% of employees would recommend working at Thermo Fisher Scientific with the overall rating of 4.0 out of 5. Employees also rated Thermo Fisher Scientific 3.8 out of 5 for Company Culture, 3.7 for Rewards You Receive, 3.5 for Growth Opportunities and 3.8 for support you get.
What is the highest paying job at Thermo Fisher Scientific?
According to our data, the highest paying job at Thermo Fisher Scientific is a Senior Application Architect at $209,000 annually. Browse Thermo Fisher Scientific Salaries by Job Profile.
What is the lowest paying job at Thermo Fisher Scientific?
According to our data, the lowest paying job at Thermo Fisher Scientific is a Quality Control Specialist at $18,000 annually. Browse Thermo Fisher Scientific Salaries by Job Profile.
What are the pros and cons of working at Thermo Fisher Scientific?
According to reviews on CareerBliss, employees commonly rated the pros of working at Thermo Fisher Scientific to be Company Culture, Growth Opportunities, People You Work With and Person You Work For, and no cons.
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue of approximately $40 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, increasing productivity in their laboratories, improving patient health through diagnostics or the development and manufacture of life-changing therapies, we are here to support them. Our global team of more than 100,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon and PPD. For more information, please visit www.thermofisher.com.The company is tremendously good. Very good business policy and wonderful career growth. However I have rated the aspects based on the division I am into.
Dislikes
Based on the division I am working for, it is the worst place for anyone. Most of the employees, from lower to higher level leaves the job within few years. The management is worse than ever. Micromanaging, Revenge minded manager, poor empathy culture, and mostly biased nature of manager is pulling down the enthusiasm for everyone. Don't believe my words, join our division and look for yourself!
Work Details
It was good until the recent person who has been promoted as manager. He is the worst. Tyrant, Revenge minded, Biased recruiting, No help to employees who are "not South Indian", manipulate the HR, and many more. Most of the tenured ppl are re
Yes, there are politics, and cultures within departments/teams that don't fit the utopian (a situation you'll find in every workplace) environment. However, the people, goals, and daily tasks have always provided the satisfaction of having made a meaningful effort towards a better future (for myself, and others).
The biggest issue with the company though is that it is so large, the little teams/departments are very often left out of consideration for the benefits offered to the (culturally) more meaningful income producing teams/departments. These teams are often not considered when policy's and decisions are made that benefit the 'normal' employees, but frequently hurt (making both personal, and professional life) the ones left out in spite of the company wide 4i "values."
NaCl80 gKCl2 gNa2HPO414.4 gNaH2PO42.4 gDeionized water900 mLpH to 7.0 with NaOHDeionized waterto 1000 mL
Sample preparation buffers
RIPA buffer: 25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS (100 mL)
NaCl0.88 gNP-401 gSodium deoxycholate1 g10% SDS1 mL1 M Tris-HCl, pH 7.62.5 mLDeionized waterto 100 mLProtease Inhibitor Tablet (Cat. No. A32965)2 tablets
SDS Sample buffer (Laemmli buffer): 63 mM Tris HCl, 10% Glycerol, 2% SDS, 0.0025% Bromophenol Blue, pH 6.8 (10 mL)
Recipe for 2X buffer stock:
0.5 M Tris-HCl pH 6.82.5 mLGlycerol2 mL10% (w/v) SDS4 mL0.1% (w/v) Bromophenol Blue0.5 mLDeionized waterto 10 mL
The buffer is stable for 6 months when stored at 4°C.
LDS Sample Buffer: 106 mM Tris HCl, 141 mM Tris Base, 2% LDS, 10% Glycerol, 0.51 mM EDTA, 0.22 mM SERVA Blue G250, 0.175 mM Phenol Red, pH 8.5.
Recipe for 4X buffer stock:
Tris HCl0.666 gTris Base0.682 gLDS0.800 gEDTA0.006 gGlycerol4 gSERVA Blue G250 (1% solution)0.75 mLPhenol Red (1% solution)0.25 mLDeionized waterto 10 mL
The buffer is stable for 6 months when stored at 4°C. Do not use acid or base to adjust pH.
Electrophoresis running buffers
Tris-Glycine SDS Running Buffer: 25 mM Tris Base, 192 mM Glycine, 0.1% SDS, pH 8.3.
Recipe for 10X buffer stock:
Tris Base29 gGlycine144 gSDS10 gDeionized waterto 1000 mL
Tris-Glycine Native Running Buffer: 25 mM Tris Base, 192 mM Glycine, pH 8.3.
Recipe for 10X buffer stock:
Tris Base29 gGlycine144 gDeionized waterto 1000 mL
MOPS SDS Running Buffer: 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7.
Recipe for 20X buffer stock:
MOPS104.6 gTris Base60.6 gSDS10 gEDTA3.0 gDeionized waterto 500 mL
Do not use acid or base to adjust pH.
MES SDS Running Buffer: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3.
Recipe for 20X buffer stock:
MES97.6 gTris Base60.6 gSDS10 gEDTA3.0 gDeionized waterto 500 mL
Do not use acid or base to adjust pH.
Tricine SDS Running Buffer: 100 mM Tris Base, 100 mM Tricine, 0.1% SDS, pH 8.3.
Recipe for 10X buffer stock:
Tris Base121 gTricine179 gSDS10 gDeionized waterto 1000 mL
The buffer is stable for 6 months when stored at room temperature.
Do not use acid or base to adjust pH.
Tris-Glycine Transfer Buffer: 12 mM Tris Base, 96 mM Glycine, pH 8.3.
Recipe for 25X buffer stock:
Tris Base18.2 gGlycine90 gDeionized waterto 500 mL
Bis-Tris Transfer Buffer: 25 mM Bicine, 25 mM Bis-Tris (free base), 1 mM EDTA, pH 7.2.
Recipe for 20X buffer stock:
Bicine10.2 gBis-Tris (free base)13.1 gEDTA0.75 gDeionized water125 mL
The buffer is stable for 6 months when stored at 4°C.
Do not use acid or base to adjust pH.
Tris-buffered saline with Tween 20 (TBST)
10X TBS100 mLTween 201 mLDeionized waterto 1000 mL
Phosphate buffered saline with Tween 20 (PBST)
10X TBS100 mLTween 201 mLDeionized waterto 1000 mL
Blocking and stripping buffers recipes
Nonfat dry milk2.5 gTBST or PBSTUp to 50 mLFilter to remove particulates
BSA1.5 gTBST or PBSTUp to 50 mLFilter to remove particulates
0.5 M Tris HCl, pH 6.812.5 mL10% SDS20 mL2-mercaptoethanol0.8 mLDeionized water67.5 mL
Recipes with SureCast reagents
The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.
*Add this last and mix well just before the gel is to be poured
Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.
*Add this last and mix well just before the gel is to be poured
Recipes with standalone reagents
Stock solutions
Prepare the following stock solutions: all solutions can be stored at room temperature.
50% Acrylamide/BIS (29:1)
- 48.3 g Acrylamide
- 1.7 g BIS
Store up to two months in a dark glass bottle.Separating Gel Buffer (1 M Tris-HCl, pH 8.8)
- Add 30.3 g Tris to 150 mL water
- Adjust pH 8.8 with HCL
- Add 11.4 g Tris to about 150 mL water
- Adjust to pH 6.8 with HCl
- 100 mg Ammonium Persulfate
Separating gel
The following recipes are for approximately 25 mL of separating gel, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.
Solution6% Gel8% Gel10% Gel12% Gel14% Gel16% Gel18% Gel20% Gel50% Acrylamide/BIS3.0 mL4.0 mL5.0 mL6.0 mL7.0 mL8.0 mL9.0 mL10.0 mLSeparating Gel Buffer9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL10% SDS250 µL250 µL250 µL250 µL250 µL250 µL250 µL250 µL50% Sucrose*4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mLWater7.8 mL6.8 mL5.8 mL4.8 mL3.7 mL2.7 mL1.7 mL750 µLTEMED**6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µLCatalyst**625 µL625 µL625 µL625 µL625 µL625 µL625 µL625 µL
*Optional but recommended because it makes it easy to form a good interface between the separating gel and the overlay. If omitted, increase the amount of water added to make up for the volume of the sucrose solution (increase the water by 4.0 mL for the above tables).
**Add these last and mix well just before the gel is to be poured.
Stacking gel
Following recipe is for 4% Stacking Gel (12.5 mL)
Solution4%50% Acrylamide/BIS1.0 mLStacking Gel Buffer4.2 mL10% SDS125 µLWater6.3 mLTEMED*5.0 µLCatalyst*1.0 mL