How does the letter E appear when viewing through the ocular lenses if it appears the correct way on the slide?

Name_________________________________________________

1. Examine your microscope. Familiarize yourself with the parts of the microscope.

The magnification written on the ocular lens (eyepiece) is _____ The magnification written on: the scanning objective _____ x

the low power objective is ______x the high power objective is ______x

2. The total magnification using the lenses can be determined by multiplying the objective lens with the ocular lens. What is the total magnification of an item viewed with the:

LOW power objective. _____ The HIGH POWER _______ The SCANNING _______

3. Examine the diaphragm (underneath the stage).
The numbers on the edge of it range from ONE to ___________

4. Look into the eyepiece, twist it left and right. Notice the line inside that moves as you twist. (Some microscopes do not have this, see if you can find one that does in the room).

What do you think this is for? _____________________________________________

5. Place the slide of the "letter e" on the stage so that the letter is over the hole and is right side up.

A) Use the scanning objective to view the letter and use the coarse knob to focus.

B) Repeat on the low power objective, also using the coarse knob to focus. You may need to recenter your e.

C) Finally, switch to high power. Remember at this point, you should only use the FINE adjustment knob.

**If you do steps above correctly, you should only part of the "e" on high power**

Draw the "e" as it appears at each magnification. Drawings should be drawn to scale and you should note the orientation of the e in the viewing field (is it upside down or right side up?)

Have your partner push the slide to the left while you view it through the lens.

Which direction does th E appear to move? __________________

6. Random Specimens

Choose 2 specimens from the box of "common things". Use the circles below to sketch your specimens under SCANNING and LOW power. You may practice focusing with the high power, but you do not need to sketch it on high power. Exercise caution, some slides say that high power is not possible because the slide is too thick. Label your specimens from the name written on the slide.

Obtain a slide with three different colored threads on it. View the slide under scanning and then low power. You should note that you could only focus on one colored thread at one time. Figure out which thread is on top by lowering your stage all the way, then slowly raising it until the thread comes into focus. The first thread to come into focus is the one on top.

Which color thread is on top? _____________ Which color thread is in the middle? ______________

Which color thread is on the bottom? ____________

8. Answer true or false to each of the statements

__________ On high power, you should use the coarse adjustment knob. __________ The diaphragm determines how much light shines on the specimen. __________ The low power objective has a greater magnification than the scanning objective. __________ The fine focus knob visibly moves the stage up and down. __________ Images viewed in the microscope will appear upside down. __________ If a slide is thick, only parts of the specimen may come into focus. __________ The type of microscope you are using is a scanning microscope. __________ For viewing, microscope slides should be placed on the objective. __________ In order to switch from low to high power, you must rotate the revolving nosepiece. __________ The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

9. (Mini-Essay) One of the most challenging tasks in this exercise is focusing using the high power objective. If your lab partner says they can't find the "e" on high power, what suggestions would you make to help her learn to use the microscope. Be specific and clear and answer this question in a complete sentence.

While looking through the microscope, move the slide to the left, notice which way the letter “e” moved. When you move the slide to the left on the stage, what direction does the image appear to move? The slide moves to the right.

When the letter E slide is viewed with the microscope it appears to move?

– The letter “e” – The viewing of this familiar letter will provide practice in orienting the slide and using the objective lenses. The letter appears upside down and backwards because of two sets of mirrors in the microscope.

What happened to the orientation of the letter E when viewing it under the microscope?

This slide was set up with the letter in the correct orientation. Notice that it appears upside down when viewed under the microscope. This is a picture of the letter “e” shown at 100X. Notice, that as you increase the power of the lens, your field of view gets smaller.

Why is the letter E inverted when viewed under the microscope? The letter appears upside down and backwards because of two sets of mirrors in the microscope. This means that the slide must be moved in the opposite direction that you want the image to move.

When you move the slide to the left in which direction does the image appear to move?

A specimen that is right-side up and facing right on the microscope slide will appear upside-down and facing left when viewed through a microscope, and vice versa. Similarly, if the slide is moved left while looking through the microscope, it will appear to move right, and if moved down, it will seem to move up.

How is the letter E on the slide oriented when you see it under low or high power magnification?

Compare the orientation of the letter “e” as viewed through the microscope with the letter “e” viewed with the naked eye on the slide. This demonstrates that in addition to being magnified the image is inverted. As magnification increases: Field of View: decreases (see table following from page 12).

How will Letter E appear in the mirror?

Answer: it will appear backwards or reversed, facing the wrong direction. Like Ǝ or ɘ. This is because light in the mirror is reflected backwards.

What happens to the orientation of the E in the field of view?

Terms in this set (19) Describe the orientation of the letter “e” as it appears through the ocular lenses. The image of the “e” appears backward and upside down. Under which objective lens is the field of view largest?

Why does microscope invert the image?

The reason compound microscopes invert images lies in the focal length of the objective lens. The image focused by the lens crosses before the eyepiece further magnifies what the observer sees, and the objective lens inverts the image because of the lens’ curvature.

How do you move left right up and down under a microscope?

– How do you move left, right, up, and down under the microscope? Why is this reversed than normal? o When you move left under a microscope it moves right, and when you move up it moves down because everything is processed upside down and backwards in our brain.

What happens to the image if you try to magnify it using 40x or 100x?

5. What happens to your image if you try to magnify it using 40x or 100x? It could blow up your iage if you do not adjust the stage accordingly.

What did you notice about the letter E when you increase in magnification?

How does the letter E as seen through the microscope differ?

The image is inverted because the converging lenses in the microscope cause the image to invert. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? It is inverted, not solid, has rough edges, and shows the texture of the paper. This is because the image is inverted.

When I moved the letter E to the left on microscope stage which way did the image of the E move?

As you move the slide toward the right of the stage, to which direction does the image of the “e” move when viewed through the microscope? To the left. You just studied 19 terms!

How does increasing the magnification affect the field of view?

In short, as magnification increases, the field of view decreases. When looking through a high power compound microscope it can be difficult to determine what you will see through the eyepieces at different magnifications.

Does a microscope flip an image?

Microscopes invert images which makes the picture appear to be upside down. The reason this happens is that microscopes use two lenses to help magnify the image. Some microscopes have additional magnification settings which will turn the image right-side-up.

Which type of image is formed by microscope?

LAB 9: THE COMPOUND MICROSCOPE An objective forms a real inverted image of an object, which is a finite distance in front of the lens. This image in turn becomes the object for the ocular, or eyepiece. The eyepiece forms the final image which is virtual, and magnified.

What happens to the image when you move the microscope slide away from you?

When you move a slide on the microscope stage away from you, in what direction does the object seen through the eyepiece move? When you move a slide away from you, the object appears to move towards you.

What parts of the microscope were moved?

PARTS OF THE MICROSCOPE

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