J Cell Biol. 1970 May 1; 45(2):
321–333. Article Zonal Centrifugation Evidence for Multiple Cellular Sources AbstractPostnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose—0.02 M KCl gradients. Zonal fractions were analyzed for protein, RNA, cytochrome oxidase, and up to six acid hydrolases. The results indicate the presence of two groups of lysosome-like particles. One group contributes approximately 95% of the cathepsin D and acid phosphatase activity and 75% of the acid ribonuclease, β-glucuronidase, and arylsulfatase activity in muscle. It is characterized by a modal equilibrium density of 1.18 that is decreased by starvation, but is not shifted by dextran-500 or Triton WR-1339. The second group has a higher proportion of acid ribonuclease, β-glucuronidase, and arylsulftase; the equilibrium density can be shifted by dextran-500 and Triton WR-1339. It is suggested that this group of lysosomes is derived from macrophages and other connective tissue cells, whereas the former group represents lysosome-like particles from muscle cells. Full TextThe Full Text of this article is available as a PDF (886K). Selected ReferencesThese references are in PubMed. This may not be the complete list of references from this article.
Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press Do muscles contain lysosomes?Biological activity of lysosomes
Lysosomes are present in all eukaryotic cells. Their specific activity appears to differ between tissues and species, and is age-dependent [40]. In spleen and liver, lysosomal activity is reported to be high, in differentiated muscle tissue it is low [12].
What organelles are in muscle cells?To meet this energy demand, muscle cells contain mitochondria. These organelles, commonly referred to as the cell's “power plants,” convert nutrients into the molecule ATP, which stores energy.
What molecules are in muscle cells?Muscle cells are long, thin tubes divided into segments, each containing filamentary molecules called actin and myosin. The two molecules interpenetrate each other like two toothbrush heads pushed together bristle to bristle.
Does a muscle cell have a vacuole?Gross morphological changes with vacuole formation in muscle cells during the recovery period have been reported and it has been suggested that this is the cause of the delayed force recovery.
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