Inside the mitochondrion is a certain type of DNA. That's different in a way from the DNA that's in the nucleus. This DNA is small and circular. It has only 16,500 or so base pairs in it. And it encodes different proteins that are specific for the mitochondrial. Now, remember those pathways that are within the mitochondrion for producing energy. Some of the enzymes in those pathways, and some of the proteins that are needed to function in those pathways, are produced by the mitochondrial DNA. The mitochondrial DNA is critically important for many of the pathways that produce energy within the mitochondria. And if there's a defect in some of those mitochondrial DNA bases, that is to say a mutation, you will have a mitochondrial disease, which will involve the inability to produce sufficient energy in things like the muscle and the brain, and the kidney. Mitochondrial DNA, unlike nuclear DNA, is inherited from the mother, while nuclear DNA is inherited from both parents. So this is very helpful sometimes in determining how a person has a certain disorder in the family. Sometimes a disease will be inherited through the mother's line, as opposed to both parents. You can tell from a pedigree or a group of family history whether or not this is a mitochondrial disease because of that. Show
 What is DNA?Deoxyribonucleic acid (DNA) carries genetic information that is used as a set of instructions for growth and development, as well as the ultimate functioning and reproduction of living organisms. It is a nucleic acid and is one of the four major types of macromolecules that are known to be essential for all forms of life1. Each DNA molecule is comprised of two biopolymer strands coiling around each other to form a double helix. These two DNA strands are called polynucleotides, as they are made of simpler monomer units called nucleotides2. Each individual nucleotide is composed of one of four nitrogen-containing nucleobases – Cytosine (C), Guanine (G), Adenine (A) or Thymine (T) – along with a sugar called deoxyribose and a phosphate group. Nucleotides are joined to one another by covalent bonds, between the phosphate of one nucleotide and the sugar of the next. This creates a chain, resulting in an alternating sugar-phosphate backbone. Nitrogenous bases of the two polynucleotide strands are bound together by hydrogen bonds, to make double stranded DNA according to strict base pairings (A to T and C to G)3. Within eukaryotic cells, DNA is organised into structures called chromosomes, with each cell having 23 pairs of chromosomes. During cell division, chromosomes are duplicated through the process of DNA replication, as long as each cell has its own complete set of chromosomes. Eukaryotic organisms such as animals, plants and fungi, store the majority of their DNA inside the cell nucleus and some of their DNA in organelles such as mitochondria4. Being located in different regions of the eukaryotic cell, there are a number of fundamental differences between mitochondrial DNA (mtDNA) and nuclear DNA (nDNA). Based on key structural and functional properties, these differences affect how they operate within eukaryotic organisms. Organization and structural differences of Mitochondrial DNA and Nuclear DNALocation → Located exclusively in the mitochondria, mtDNA contains 100-1,000 copies per somatic cell. Nuclear DNA is located within the nucleus of every eukaryotic cell (with some exceptions such as nerve and red blood cells) and usually only has two copies per somatic cell5. Structure → Both types of DNA are double stranded. However, nDNA has a linear, open ended structure that is enclosed by a nuclear membrane. This differs from mtDNA, which usually has a closed, circular structure and is not enveloped by any membrane Genome sizes → Both mtDNA and nDNA have their own genomes but are very different sizes. In humans, the size of the mitochondrial genome consists of only 1 chromosome that contains 16,569 DNA base pairs. The nuclear genome is significantly larger than the mitochondrial, consisting of 46 chromosomes that contain 3.3 billion nucleotides. Gene encoding → The singular mtDNA chromosome is much shorter than the nuclear chromosomes. It contains 36 genes that encode for 37 proteins, all of which are specific proteins used in the metabolic processes mitochondria undertake (such as citrate acid cycle, ATP synthesis and fatty acid metabolism). The nuclear genome is much larger, with 20,000-25,000 genes coding for all the proteins required for its function, which also includes mitochondrial genes. Being semi-autonomous organelles, mitochondrion cannot code for all of its own proteins. However, they can encode for 22 tRNAs and 2 rRNAs, which nDNA lacks the capability to do. Functional differencesTranslation process → The translation process between nDNA and mtDNA can vary. nDNA follows the universal codon pattern, however this is not always the case for mtDNA. Some mitochondrial coding sequences (triplet codons) do not follow the universal codon pattern when they are translated into proteins. For example, AUA codes for methionine in mitochondrion (not Isoleucine). UGA also codes for tryptophan (not a stop codon as in mammalian genome)6. Transcription process → Gene transcription within mtDNA is polycistronic, meaning an mRNA is formed with sequences that code for many proteins. For nuclear gene transcription the process is monocistronic, where the mRNA formed has sequences coding for
only a single protein8. Genome inheritance → Nuclear DNA is diploid, meaning it inherits DNA both maternally and paternally (23 chromosomes from each of the mother and father). However, mitochondrial DNA is haploid, with the single chromosome being inherited down the maternal side and does not undergo genetic recombination9. Mutation rate → As nDNA undergoes genetic recombination, it is a
shuffle of the parent’s DNA and is therefore altered during inheritance from the parents to their offspring. However, as mtDNA is only inherited from the mother, there is no alteration during transmission, meaning any DNA changes come from mutations. The mutation rate in mtDNA is much higher than in nDNA, which is normally less than 0.3%10. Differences in the application of mtDNA and nDNA within scienceThe different structural and functional properties of mtDNA and nDNA, have led to differences in their applications within science. With its significantly greater mutation rate, mtDNA has been utilized as a powerful tool for tracking ancestry and lineage through females (matrilineage). Methods have been developed that are used to track the ancestry of many species back through hundreds of generations and has become the mainstay of phylogenetics and evolutionary biology. Due to the higher mutation rate, mtDNA evolves much faster than nuclear genetic markers11. There are many variations among the codes used by mtDNA that arise from mutations, many of which are not harmful to their organisms. Utilizing this greater mutation rate and these non-harmful mutations, scientists determine mtDNA sequences and compare them from different individuals or species. A network of relationships among these sequences is then constructed which provides an estimate of the relationships among either the individuals or species from which the mtDNA was taken. This gives an idea of how closely and distantly related each is – the more mtDNA mutations that are the same in each of their mitochondrial genomes, the more related they are. Due to the lower mutation rate of nDNA, it has a more restricted application in the field of phylogenetics. However, given the genetic instructions it holds for the development of all living organisms, scientists have recognised its use in forensics. Every single person has a unique genetic blueprint, even identical twins12. Forensic departments are able to use polymerase chain reaction (PCR) techniques, using nDNA, to compare samples in a case. This involves using small amounts of nDNA to make copies of targeted regions called short tandem repeats (STRs) on the molecule13. From these STRs, a ‘profile’ is obtained from items of evidence, which can then be compared to known samples taken from the individuals involved in the case. Human mtDNA can also be used to help identify individuals using forensics, however unlike nDNA, it is not specific to one individual but can be used in combination with other evidence (such as anthropological and circumstantial
evidence) to establish identification. Because mtDNA has a greater number of copies per cell than nDNA, it has the ability to identify much smaller, damaged or degraded biological samples14. The greater number of mtDNA copies per cell than nDNA, also makes it is possible to obtain a DNA match with a living relative, even if numerous maternal generations separate them from the skeletal remains of a relative. Tabular comparison of key differences between Mitochondrial and Nuclear DNA
Loading... Cite What is difference between mitochondrial DNA and nuclear DNA?Mitochondrial DNA, unlike nuclear DNA, is inherited from the mother, while nuclear DNA is inherited from both parents. So this is very helpful sometimes in determining how a person has a certain disorder in the family. Sometimes a disease will be inherited through the mother's line, as opposed to both parents.
How does mitochondrial DNA differ from nuclear DNA quizlet?Nuclear DNA is DNA in cell nuclei and is responsible for the majority of functions that cells carry out. Mitochondrial DNA is associated with animals, plants and fungi, and comes from their own DNA and is quite different in form than nuclear DNA. Whereas human nuclear DNA is linear, mitochondrial DNA is circular.
What is the major difference between eukaryotic nuclear DNA and mitochondrial DNA?Differences in the application of mtDNA and nDNA within science. What is difference between nuclear genome and mitochondrial genome?The mitochondrial genome is circular, whereas the nuclear genome is linear (Figure 3). The mitochondrial genome is built of 16,569 DNA base pairs, whereas the nuclear genome is made of 3.3 billion DNA base pairs. The mitochondrial genome contains 37 genes that encode 13 proteins, 22 tRNAs, and 2 rRNAs.
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How is mitochondrial DNA different from nuclear DNA?
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