Is thermo fisher a good company to work for

Thermo Fisher Scientific has an overall rating of 4.0 Average Rating out of 5, based on over 109 Thermo Fisher Scientific Review Ratings left anonymously by Thermo Fisher Scientific employees, which is 3% higher than the average rating for all companies on CareerBliss. 99% of employees would recommend working at Thermo Fisher Scientific.

Does Thermo Fisher Scientific pay their employees well?

Thermo Fisher Scientific employees earn $61,000 annually on average, or $29 per hour, which is 8% lower than the national salary average of $66,000 per year. 64 Thermo Fisher Scientific employees have shared their salaries on CareerBliss. Find Thermo Fisher Scientific Salaries by Job Title.

How satisfied are employees working at Thermo Fisher Scientific?

99% of employees would recommend working at Thermo Fisher Scientific with the overall rating of 4.0 out of 5. Employees also rated Thermo Fisher Scientific 3.8 out of 5 for Company Culture, 3.7 for Rewards You Receive, 3.5 for Growth Opportunities and 3.8 for support you get.

What is the highest paying job at Thermo Fisher Scientific?

According to our data, the highest paying job at Thermo Fisher Scientific is a Senior Application Architect at $209,000 annually. Browse Thermo Fisher Scientific Salaries by Job Profile.

What is the lowest paying job at Thermo Fisher Scientific?

According to our data, the lowest paying job at Thermo Fisher Scientific is a Quality Control Specialist at $18,000 annually. Browse Thermo Fisher Scientific Salaries by Job Profile.

What are the pros and cons of working at Thermo Fisher Scientific?

According to reviews on CareerBliss, employees commonly rated the pros of working at Thermo Fisher Scientific to be Company Culture, Growth Opportunities, People You Work With and Person You Work For, and no cons.

The company is tremendously good. Very good business policy and wonderful career growth. However I have rated the aspects based on the division I am into.

Dislikes

Based on the division I am working for, it is the worst place for anyone. Most of the employees, from lower to higher level leaves the job within few years. The management is worse than ever. Micromanaging, Revenge minded manager, poor empathy culture, and mostly biased nature of manager is pulling down the enthusiasm for everyone. Don't believe my words, join our division and look for yourself!

Work Details

It was good until the recent person who has been promoted as manager. He is the worst. Tyrant, Revenge minded, Biased recruiting, No help to employees who are "not South Indian", manipulate the HR, and many more. Most of the tenured ppl are re

Yes, there are politics, and cultures within departments/teams that don't fit the utopian (a situation you'll find in every workplace) environment. However, the people, goals, and daily tasks have always provided the satisfaction of having made a meaningful effort towards a better future (for myself, and others).

The biggest issue with the company though is that it is so large, the little teams/departments are very often left out of consideration for the benefits offered to the (culturally) more meaningful income producing teams/departments. These teams are often not considered when policy's and decisions are made that benefit the 'normal' employees, but frequently hurt (making both personal, and professional life) the ones left out in spite of the company wide 4i "values."

NaCl80 gKCl2 gNa2HPO414.4 gNaH2PO42.4 gDeionized water900 mLpH to 7.0 with NaOHDeionized waterto 1000 mL

Sample preparation buffers

RIPA buffer: 25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS (100 mL)

NaCl0.88 gNP-401 gSodium deoxycholate1 g10% SDS1 mL1 M Tris-HCl, pH 7.62.5 mLDeionized waterto 100 mLProtease Inhibitor Tablet (Cat. No. A32965)2 tablets

SDS Sample buffer (Laemmli buffer): 63 mM Tris HCl, 10% Glycerol, 2% SDS, 0.0025% Bromophenol Blue, pH 6.8 (10 mL)

Recipe for 2X buffer stock:

0.5 M Tris-HCl pH 6.82.5 mLGlycerol2 mL10% (w/v) SDS4 mL0.1% (w/v) Bromophenol Blue0.5 mLDeionized waterto 10 mL

The buffer is stable for 6 months when stored at 4°C.

LDS Sample Buffer: 106 mM Tris HCl, 141 mM Tris Base, 2% LDS, 10% Glycerol, 0.51 mM EDTA, 0.22 mM SERVA Blue G250, 0.175 mM Phenol Red, pH 8.5.

Recipe for 4X buffer stock:

Tris HCl0.666 gTris Base0.682 gLDS0.800 gEDTA0.006 gGlycerol4 gSERVA Blue G250 (1% solution)0.75 mLPhenol Red (1% solution)0.25 mLDeionized waterto 10 mL

The buffer is stable for 6 months when stored at 4°C. Do not use acid or base to adjust pH.

Electrophoresis running buffers

Tris-Glycine SDS Running Buffer: 25 mM Tris Base, 192 mM Glycine, 0.1% SDS, pH 8.3.

Recipe for 10X buffer stock:

Tris Base29 gGlycine144 gSDS10 gDeionized waterto 1000 mL

Tris-Glycine Native Running Buffer: 25 mM Tris Base, 192 mM Glycine, pH 8.3.

Recipe for 10X buffer stock:

Tris Base29 gGlycine144 gDeionized waterto 1000 mL

MOPS SDS Running Buffer: 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7.

Recipe for 20X buffer stock:

MOPS104.6 gTris Base60.6 gSDS10 gEDTA3.0 gDeionized waterto 500 mL

Do not use acid or base to adjust pH.

MES SDS Running Buffer: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3.

Recipe for 20X buffer stock:

MES97.6 gTris Base60.6 gSDS10 gEDTA3.0 gDeionized waterto 500 mL

Do not use acid or base to adjust pH.

Tricine SDS Running Buffer: 100 mM Tris Base, 100 mM Tricine, 0.1% SDS, pH 8.3.

Recipe for 10X buffer stock:

Tris Base121 gTricine179 gSDS10 gDeionized waterto 1000 mL

The buffer is stable for 6 months when stored at room temperature.
Do not use acid or base to adjust pH.

Tris-Glycine Transfer Buffer: 12 mM Tris Base, 96 mM Glycine, pH 8.3.

Recipe for 25X buffer stock:

Tris Base18.2 gGlycine90 gDeionized waterto 500 mL

Bis-Tris Transfer Buffer: 25 mM Bicine, 25 mM Bis-Tris (free base), 1 mM EDTA, pH 7.2.

Recipe for 20X buffer stock:

Bicine10.2 gBis-Tris (free base)13.1 gEDTA0.75 gDeionized water125 mL

The buffer is stable for 6 months when stored at 4°C.
Do not use acid or base to adjust pH.

Tris-buffered saline with Tween 20 (TBST)

10X TBS100 mLTween 201 mLDeionized waterto 1000 mL

Phosphate buffered saline with Tween 20 (PBST)

10X TBS100 mLTween 201 mLDeionized waterto 1000 mL

Blocking and stripping buffers recipes

Nonfat dry milk2.5 gTBST or PBSTUp to 50 mLFilter to remove particulates

BSA1.5 gTBST or PBSTUp to 50 mLFilter to remove particulates

0.5 M Tris HCl, pH 6.812.5 mL10% SDS20 mL2-mercaptoethanol0.8 mLDeionized water67.5 mL

Recipes with SureCast reagents

The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.

*Add this last and mix well just before the gel is to be poured

Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.

*Add this last and mix well just before the gel is to be poured

Recipes with standalone reagents

Stock solutions

Prepare the following stock solutions: all solutions can be stored at room temperature.

50% Acrylamide/BIS (29:1)

• 48.3 g Acrylamide
• 1.7 g BIS

• Add 30.3 g Tris to 150 mL water
• Adjust pH 8.8 with HCL

• Add 11.4 g Tris to about 150 mL water
• Adjust to pH 6.8 with HCl

• 100 mg Ammonium Persulfate

Separating gel

The following recipes are for approximately 25 mL of separating gel, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.

Solution6% Gel8% Gel10% Gel12% Gel14% Gel16% Gel18% Gel20% Gel50% Acrylamide/BIS3.0 mL4.0 mL5.0 mL6.0 mL7.0 mL8.0 mL9.0 mL10.0 mLSeparating Gel Buffer9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL10% SDS250 µL250 µL250 µL250 µL250 µL250 µL250 µL250 µL50% Sucrose*4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mLWater7.8 mL6.8 mL5.8 mL4.8 mL3.7 mL2.7 mL1.7 mL750 µLTEMED**6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µLCatalyst**625 µL625 µL625 µL625 µL625 µL625 µL625 µL625 µL

*Optional but recommended because it makes it easy to form a good interface between the separating gel and the overlay. If omitted, increase the amount of water added to make up for the volume of the sucrose solution (increase the water by 4.0 mL for the above tables).
**Add these last and mix well just before the gel is to be poured.

Stacking gel

Following recipe is for 4% Stacking Gel (12.5 mL)

Solution4%50% Acrylamide/BIS1.0 mLStacking Gel Buffer4.2 mL10% SDS125 µLWater6.3 mLTEMED*5.0 µLCatalyst*1.0 mL

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