Samantha and charlie have been living together for the past three years. they are engaged in:

Sami befriends Lucas Horton and dates his friend Alan Harris, whom her family strongly opposes. Sami's best friend Jamie overhears Alan talk about being in love with Carrie Brady, but keeps this from Sami. Frustrated by his unrequited love for Carrie, Alan rapes Sami. Sami confides in Lucas, but without corroborating proof, the press vilifies Sami as a liar once the news gets out. Alan tries to rape her again, and she stops him by shooting him in the groin. Distraught, Lucas manages to comfort Sami; and they have sex. She still, however, has a crush on Austin and drugs him into bed so he would believe she was Carrie. After her divorce from Roman, Marlena becomes vulnerable and is possessed by the devil. While possessed, Marlena tells Sami to seduce Austin. She tries, but Austin rejects her. Sami leaves town in February 1995.

Sami returns in July of that year and crashes Austin's wedding to Carrie claiming that she is carrying his child. After a rough teen pregnancy, Sami gives birth to William Robert Reed on November 16, 1995. Sami hides the fact that Will is actually Lucas' son, and passes him off as Austin's. Austin and Sami are engaged and plan to wed. However, he leaves her at the altar after Carrie uncovers the truth about Will's paternity. Sami renames her son William Reed Roberts and leaves town. She returns and befriends Franco Kelly (Victor Alfieri). They become engaged; Franco is using her to get a green card and stay in the country. He is murdered on their wedding day. Sami sees the body and faints near it. Lucas's mother Kate Roberts frames Sami for the murder so that she will go to jail and Lucas can have full custody of Will. Sami is convicted and sentenced to death for the murder. In the middle of her execution via injection, Lucas confesses to the murder in an attempt to save her life. Unbeknownst to Lucas, Roberto signed a false confession on his death bed which leads to a stay of execution from the governor. Sami is freed and falls in love with Brandon Walker, who helps her regain full custody of Will. Brandon and Sami are married briefly. However, her lies and schemes end the marriage. Sami has an accident at the DiMera mansion and falls through the window. Lucas helps her recover and they fall in love. The become engaged, much to Kate's chagrin. Kate drugs Sami and places her in Brandon's bed the night before her marriage to Lucas in a bid to end the engagement. Lucas leaves Sami.

Sami's sorely compromised psychological state deteriorates to such a degree that Sami begins to work for Tony DiMera, becomes a transvestite under an assumed name, "Stan", and sells illicit drugs to a pain-wracked John Black, all out of a base-born, suppositions need for revenge. Attempting to redeem herself, Sami convinces Lucas that Kate had set her up; that she was never unfaithful. The two reunite and agree to marry once again.

Lucas believes that Sami has changed for the better, but Kate reveals Sami's misdeeds as "Stan," and Lucas calls off the wedding. Austin Reed returns to Salem and befriends Sami. Carrie Brady also returns after ending her relationship with Mike Horton. Lucas and Austin, now business rivals, attempt to buy out Carrie's company. Lucas backs out, finding out that Carrie runs the entire company, and that once again, Sami manipulated him. Austin and Carrie fall back in love and plan their future together when Carrie learns that Austin's company has taken over High-style. Carrie dates Lucas on the rebound. Austin does the same with Sami, and both couples become engaged.

Sami's relationship to Austin is tested however, when E.J. Wells, an English race car driver, moves into the apartment next to theirs. When Sami and Austin's wedding ends in disaster, EJ and Sami kiss for the first time. Carrie ends her relationship with Lucas and remarries Austin. They leave to start a life together in Switzerland.

Sami turns to E.J., and the two begin dating. Sami then discovers that not only is c sleeping with Kate but that he is also Stefano's son. Sami and Lucas rekindle their love in spite of Kate, and the two decide to take a road trip. Their car breaks down in a snow storm, and they seek shelter in an abandoned cabin where they make love. Weighed down by snow, the crumbling ceiling caves in. Lucas is trapped, Sami runs to get help, meets up with E.J. in a snow drift, and asks him to help her. Revealing himself as a true DiMera, E.J. agrees to help Sami only if she has sex with him. In subsequent weeks, E.J. continues to taunt Sami with reminders of the deal they made that night. Sami tells Lucas about her pregnancy and they get married; the night of their wedding, he learns about E.J. raping her. Her pregnancy was full of speculation about who the father of her unborn twins were. She later gives birth to John "Johnny" Roman Roberts (later legally changed to John Roman DiMera) and Alice "Allie" Caroline Horton. It was revealed after a paternity test that E.J. was Johnny's father and Allie was Lucas' daughter. Sami makes the painful decision to end her marriage to Lucas and marry E.J. to end the Brady-DiMera feud. At her wedding to E.J., Lucas, Marlena and Kate attempt to shoot E.J. Lucas is arrested and sent to prison, and Will leaves for Switzerland. It is later revealed that Will was the actual shooter and Lucas went to prison to protect his son.

E.J. tries to take custody of Johnny. But immigration wants to deport E.J., and Sami helps him by moving into the DiMera mansion with Johnny to show to immigration that they are a happily married couple. They have sex, and Lucas, released on house arrest, sees them at it. Although Sami ends her marriage to E.J., Lucas doesn't forgive her. E.J. moves on to Nicole Walker, and Sami finds out she is pregnant again. She doesn't tell E.J., and after witnessing a murder, she goes into witness protection. She befriends her guard, Rafe Hernandez, and gives birth to a daughter. Nicole miscarries E.J.'s child but pretends to still be pregnant and secretly illegally adopts a baby girl to pass off to E.J. as their own. Nicole then finds out that Sami's baby daughter is actually E.J.'s and she secretly switches the two baby girls, so that E.J. will be raising a child that is biologically his. Nicole's switch works and she begins raising Sami's child, Sydney DiMera, as her own. Sami is in love with Rafe, and together they raise the baby, named Grace DiMera, believing Sami gave birth to her. Grace contracts meningitis and dies, and Sami confesses to E.J. that Grace was "his", and E.J. is furious. Eventually, the baby switch is revealed and Sami is reunited with Sydney. But for revenge against Sami, E.J. kidnaps Sydney and makes everyone think she is dead. But he falls back in love with Sami and returns Sydney to her, pretending to be the hero. E.J. and Sami reconnect, and after her relationship with Rafe ends, she becomes engaged to E.J. However, at the wedding, Rafe presents evidence to Sami that E.J. was behind Sydney's kidnapping. She leaves E.J. for Rafe, and discovers that he had a plan to kidnap both his children and take them away forever. To stop him, she shoots him in the head. Rafe proposes to her while E.J. narrowly escapes death. He later wins full custody of the children and Sami marries Rafe. Johnny is diagnosed with eye cancer and she regains joint custody. After allowing Johnny and Sydney back into their mother's life, Stefano and E.J. switch Rafe with an impostor whose face they surgically alter so he can pose as Rafe's double.

The DiMeras' schemes are eventually revealed, and after some months, the real Rafe is reunited with Sami. During a family get-together for John Black, Johnny goes missing and Sami rushes to the DiMera mansion insisting that E.J. has him. They see in a news report that Johnny has "died" and in their grief, have sex, and Will sees them at it! However, the news report turns out to be false and Johnny is found alive and well. Will is angry with his mom for being unfaithful to Rafe, and when Rafe finds out about it Sami's marriage to Rafe ends. Lucas returns to town and Sami and Lucas reconnect. They are taken aback when Will reveals to them that he is gay. Sami initially runs out on Will, but she soon comes round to support him. Stefano is apparently murdered, and E.J. is held responsible, and Sami decides to help prove his innocence, much to Lucas's dismay. Sami's reunion with Lucas ends. Stefano is revealed to be alive, and E.J. is cleared for murder.

Sami starts to feel torn between E.J. and Rafe. Rafe and Sami agree to give their relationship a second chance. Sami plans Gabi Hernandez and Nick Fallon's wedding, wanting to tell E.J. her decision after the wedding. However, during the wedding, it is revealed that Nick isn't the father of Gabi's unborn baby – Will is. Sami is furious with Gabi, and Rafe defends his sister, insulting Will in the process. Sami is furious and breaks up with Rafe. Sami eventually tells E.J. about Rafe and her plan to leave him. E.J. forgives her, and they reunite. Nick demands Will sign away his paternal rights to the baby. When Sami, Lucas, and E.J. try to stop him, Nick reveals that he knows Will was responsible for shooting E.J. in 2007. Will signs away his rights to avoid being prosecuted, and Sami is devastated. She and E.J. team up to stop Nick's blackmail of Will. When Stefano returns, Sami and E.J. decide to get his help to destroy evidence the police has against Will. Sami and E.J. stay together through the situation, and become engaged in April 2013.

Sami comes back to Salem to mourn Will. Whilst in Salem she discovers that Ben's father Clyde Weston was the one who had gotten EJ shot, and she also finds a letter from EJ which leads her to believe EJ might be alive. The letter contains instructions which lead Sami to a safe deposit box containing the passwords to Stefano's bank account, but she is kidnapped by Stefano's son Andre DiMera who wants the passwords from her. Sami escapes and she steals Stefano's fortune. She takes the kids around Europe as she pursues clues that EJ may still be alive. In October 2017 she learns that Ben Weston has escaped from custody, and is claiming that Will is alive, and Sami once again returns to Salem to find out the truth. In 2021, Sami begins having an affair with her ex husband Lucas Horton. Kristen DiMera finds out, and sends EJ DiMera a letter, exposing Sami. After finding out, EJ confronts Sami and kicks her to the curb. Sami is then seen being kidnapped.