Show Protein synthesis is a vital process that takes place within cells and is an essential mechanism for determining cell structure and function. The processes consist of two steps, transcription and translation. Protein Synthesis. Image Credit: Soleil Nordic/Shutterstock.com The first step involves synthesizing messenger RNA (mRNA), which then leaves the nucleus and travels into the cytoplasm where it attaches to a ribosome. At this point, the second step of translation begins where the genetic code of the mRNA molecule is read and used to create a specific protein molecule. Below, we discuss these two steps in detail. What is Step 1 of Protein Synthesis?The first stage of transcription involves the transfer of genetic information from DNA into mRNA. The purpose of this stage is to take the information stored in the DNA strand and copy it so that it can be used to create a particular protein molecule. During the phase of transcription, a strand of mRNA is synthesized to complement a specific segment of DNA. This happens in three steps. First is the step of initiation, which represents the beginning of the transcription process. Here, the enzyme RNA polymerase locates and binds to an area of the gene known are the ‘promoter’. Once the binding has occurred, this signals the DNA strands to begin unwinding, allowing the enzyme RNA polymerase to read the bases of one of its strands. Once complete, the enzyme RNA polymerase can then synthesize a strand of mRNA composed of the same sequence bases. Next, the step of elongation begins, where nucleotides are added to the strand of mRNA. Finally, once this step is complete, the final step of termination is initiated. Termination is the end of transcription, where the synthesis of the mRNA strand is completed and detaches itself from the DNA. In humans and other eukaryotes, the newly created strand of mRNA must be processed before it can continue to the second step of protein synthesis, translation. Before processing, the new mRNA is known as pre-mRNA and before leaving the nucleus as mature mRNA must go through a final stage of processing. Often, this involves steps of splicing, editing, and polyadenylation. During these steps, the pre-mRNA molecule is modified, allowing one single gene to be used to create multiple proteins. Below we look at these steps in further detail. Splicing relies on ribonucleoproteins found in the nucleus and involves the removal of regions go the genetic code, known as introns, from the pre-mRNA. This leaves the pre-mRNA with only protein-coding regions, known as exons. The second step of processing is the editing phase. This is where changes are made to some of the pre-mRNA’s nucleotides. This editing allows for different versions of a single protein molecule to exist, such as the human protein APOB, which has two forms as a result of editing and works in the body to transport lipids in the blood. The final step of processing is polyadenylation. This is where a tail of adenine bases is added to the strand of mRNA. The addition of this ‘tail’ signals the end of mRNA and also protects it from enzymes that may try to degrade it once it is exported from the nucleus. What is the 2nd Step of Protein Synthesis?Translation is the second step of protein synthesis. Once transcription and the following processing are complete, translation is initiated. This is where the newly created mRNA’s genetic code is read and used to produce protein molecules. Once mRNA leaves the nucleus it travels to a ribosome. Here, the ribosome reads the chain of codons in the strand of mRNA, and then tRNA transports the corresponding amino acids to the ribosome in the exact sequence. Each molecule of tRNA has an anticodon to the specific amino acid it carries. Each anticodon has a complementary codon for the specific amino acid. This enables tRNA to transport the correct amino acids in the right order as coded on the strand of mRNA. Once the tRNA arrives at the mRNA with the correct amino acid it temporarily binds to it and gives up its amino acid which bonds to the previously added amino acid in the polypeptide chain. This chain continues to grow until a stop codon is presented. Summary of the Steps of Protein SynthesisProtein synthesis is an essential process that happens regularly within cells. The process is used to create new proteins that are used for various vital functions in the body. The process involves two stages of transcription and translation, with the need for processing in-between the two stages. First, transcription transfers the genetic information from DNA to mRNA via initiation, elongation, and termination. Following this, the newly created strand of mRNA leaves the nucleus and attaches to a ribosome within the cytoplasm. This is where translation initiates. During this stage the genetic data is read, causing tRNA to transport the correct sequence of amino acids to the ribosome, creating a polypeptide chain. Finally, the polypeptide chain may go through the final processing to produce the finished protein molecule. Sources:
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Created by: CK-12/Adapted by Christine Miller Figure 5.7.1 How proteins are made.The Art of Protein SynthesisThis amazing artwork (Figure 5.7.1) shows a process that takes place in the cells of all living things: the production of proteinsno post. This process is called , and it actually consists of two processes — and . In cells, transcription takes place in the . During transcription, is used as a template to make a molecule of messenger RNA (). The molecule of mRNA then leaves the nucleus and goes to a in the , where translation occurs. During translation, the genetic code in mRNA is read and used to make a polypeptide. These two processes are summed up by the central dogma of molecular biology: → → . Transcription is the first part of the central dogma of molecular biology: DNA → RNA. It is the transfer of genetic instructions in DNA to mRNA. During transcription, a strand of mRNA is made to complement a strand of DNA. You can see how this happens in Figure 5.7.2. Figure 5.7.2 Transcription uses the sequence of bases in a strand of DNA to make a complementary strand of mRNA. Triplets are groups of three successive nucleotide bases in DNA. Codons are complementary groups of bases in mRNA.Transcription begins when the enzyme RNA polymerase binds to a region of a gene called the promoter sequence. This signals the DNA to unwind so the enzyme can “read” the bases of DNA. The two strands of DNA are named based on whether they will be used as a template for RNA or not. The strand that is used as a template is called the template strand, or can also be called the antisense strand. The sequence of bases on the opposite strand of DNA is called the non-coding or sense strand. Once the DNA has opened, and RNA polymerase has attached, the RNA polymerase moves along the DNA, adding RNA nucleotides to the growing mRNA strand. The template strand of DNA is used as to create mRNA through complementary base pairing. Once the mRNA strand is complete, and it detaches from DNA. The result is a strand of mRNA that is nearly identical to the coding strand DNA – the only difference being that DNA uses the base thymine, and the mRNA uses uracil in the place of thymine Processing mRNAIn , the new is not yet ready for translation. At this stage, it is called pre-mRNA, and it must go through more processing before it leaves the nucleus as mature mRNA. The processing may include splicing, editing, and polyadenylation. These processes modify the mRNA in various ways. Such modifications allow a single gene to be used to make more than one protein.
Figure 5.7.3 Pre mRNA processing. mRNA requires processing before it leaves the nucleus. Translation is the second part of the central dogma of molecular biology: RNA → Protein. It is the process in which the genetic code in is read to make a . Translation is illustrated in Figure 5.7.4. After mRNA leaves the , it moves to a , which consists of rRNA and proteins. The ribosome reads the sequence of in mRNA, and molecules of bring to the ribosome in the correct sequence. Translation occurs in three stages: Initiation, Elongation and Termination. Initiation: After transcription in the nucleus, the mRNA exits through a nuclear pore and enters the cytoplasm. At the region on the mRNA containing the methylated cap and the start codon, the small and large subunits of the ribosome bind to the mRNA. These are then joined by a tRNA which contains the anticodons matching the start codon on the mRNA. This group of molecues (mRNA, ribosome, tRNA) is called an initiation complex. Elongation: tRNA keep bringing amino acids to the growing polypeptide according to complementary base pairing between the codons on the mRNA and the anticodons on the tRNA. As a tRNA moves into the ribosome, its amino acid is transferred to the growing polypeptide. Once this transfer is complete, the tRNA leaves the ribosome, the ribosome moves one codon length down the mRNA, and a new tRNA enters with its corresponding amino acid. This process repeats and the polypeptide grows. Termination: At the end of the mRNA coding is a stop codon which will end the elongation stage. The stop codon doesn’t call for a tRNA, but instead for a type of protein called a release factor, which will cause the entire complex (mRNA, ribosome, tRNA, and polypeptide) to break apart, releasing all of the components. Figure 5.7.4 Translation takes place in three stages: Initiation, Elongation and Termination. Watch this video “Protein Synthesis (Updated) with the Amoeba Sisters” to see this process in action: Protein Synthesis (Updated), Amoeba Sisters, 2018. After a polypeptide chain is synthesized, it may undergo additional processes. For example, it may assume a folded shape due to interactions between its amino acids. It may also bind with other polypeptides or with different types of molecules, such as or . Many proteins travel to the within the to be modified for the specific job they will do.7 Summary
Protein Synthesis, Teacher’s Pet, 2014. AttributionsFigure 5.7.1 How proteins are made by Nicolle Rager, National Science Foundation on Wikimedia Commons is released into the public domain (https://en.wikipedia.org/wiki/Public_domain). Figure 5.7.2 Transcription by National Human Genome Research Institute, (reworked and vectorized by Sulai) on Wikimedia Commons is released into the public domain (https://en.wikipedia.org/wiki/Public_domain). Figure 5.7.3 Pre mRNA processing by Christine Miller is used under a CC BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/) license. Figure 5.7.4 Translation by CNX OpenStax on Wikimedia Commons is used under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0) license. ReferencesAmoeba Sisters. (2018, January 18) Protein synthesis (Updated). YouTube. https://www.youtube.com/watch?v=oefAI2x2CQM&feature=youtu.be Parker, N., Schneegurt, M., Thi Tu, A-H., Lister, P., Forster, B.M. (2016, November 1). Microbiology [online]. Figure 11.15 Translation in bacteria begins with the formation of the initiation complex. In Microbiology (Section 11-4). OpenStax. https://openstax.org/books/microbiology/pages/11-4-protein-synthesis-translation Teacher’s Pet. (2014, December 7). Protein synthesis. YouTube. https://www.youtube.com/watch?v=2zAGAmTkZNY&feature=youtu.be The process of creating protein molecules. The process by which DNA is copied (transcribed) to mRNA in order transfer the information needed for protein synthesis. The process in which mRNA along with transfer RNA (tRNA) and ribosomes work together to produce polypeptides. Cells which have a nucleus enclosed within membranes, unlike prokaryotes, which have no membrane-bound organelles. A central organelle containing hereditary material. Deoxyribonucleic acid - the molecule carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses. A large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. A large complex of RNA and protein which acts as the site of RNA translation, building proteins from amino acids using messenger RNA as a template. The jellylike material that makes up much of a cell inside the cell membrane, and, in eukaryotic cells, surrounds the nucleus. The organelles of eukaryotic cells, such as mitochondria, the endoplasmic reticulum, and (in green plants) chloroplasts, are contained in the cytoplasm. A nucleic acid of which many different kinds are now known, including messenger RNA, transfer RNA and ribosomal RNA. A class of biological molecule consisting of linked monomers of amino acids and which are the most versatile macromolecules in living systems and serve crucial functions in essentially all biological processes. The addition of a poly(A) tail to a messenger RNA. The poly(A) tail consists of multiple adenosine monophosphates. A sequence of 3 DNA or RNA nucleotides that corresponds with a specific amino acid or stop signal during protein synthesis. A small RNA molecule that participates in protein synthesis. Each tRNA molecule has two important areas: an anticodon and a region for attaching a specific amino acid. Amino acids are organic compounds that combine to form proteins. A substance that is insoluble in water. Examples include fats, oils and cholesterol. Lipids are made from monomers such as glycerol and fatty acids. A biomolecule consisting of carbon (C), hydrogen (H) and oxygen (O) atoms, usually with a hydrogen–oxygen atom ratio of 2:1. Complex carbohydrates are polymers made from monomers of simple carbohydrates, also termed monosaccharides. A membrane-bound organelle found in eukaryotic cells made up of a series of flattened stacked pouches with the purpose of collecting and dispatching protein and lipid products received from the endoplasmic reticulum (ER). Also referred to as the Golgi complex or the Golgi body. |