Use this Coomassie brilliant blue R-250 solution to stain proteins in SDS-PAGE gels. Show
Protocol OverviewStep 1: Stain gels for 1–2 hours with gentle agitation. Stain overnight or longer if needed. Step 2: Destain gels for 2 hours. Change destaining solution (1610438) multiple times (e.g., 4 washes x 30 min) until the background is less dark. Packaging Options
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Item #: FB2389 Price: $144.08 Ships directly from the manufacturer. Kit for staining protein-containing polyacrylamide gels, includes 1 L Coomassie Brilliant Blue R-250 staining solution and 2 x 1 L destaining solution; education use only See more product details This item can only be shipped to schools, museums and science centers Product DetailsCoomassie (Brilliant) Blue R-250 staining solution is the easiest way to fix and detect proteins in polyacrylamide gels. Ready-to-use solutions are packaged in 1 L sizes and eliminate the need to weigh powders or dilute solutions. Bio-Rad Item No.: 1610435EDU SpecificationsCoomassie (Brilliant) Blue R-250 staining solution is the easiest way to fix and detect proteins in polyacrylamide gels. Ready-to-use solutions are packaged in 1 L sizes and eliminate the need to weigh powders or dilute solutions.
This protocol was adapted from “Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins,” Chapter 7, in Proteins and Proteomics by Richard J. Simpson. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003. INTRODUCTIONThe most commonly used dye for visualizing proteins in SDS-PAGE gels is Coomassie Brilliant Blue R250 (CBR-250) because of its relatively high sensitivity. This protocol describes the standard CBR-250 staining method, along with a simple method for preparing stained gels for long-term storage. What does Coomassie Blue stain do in SDSCoomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent ("destaining"). This treatment allows the visualization of proteins as blue bands on a clear background.
How do I prepare a solution of Coomassie RSolutions
To prepare 1 l of a staining solution, dissolve 1 g of Coomassie R250 to 300 ml of methanol. Then add 650 ml of MQ water and 50 ml of acetic acid. Stir the solution on a magnetic stirrer for 2 h. The solution can be filtered through a Whatman No.
What is the difference between R250 and g250 Coomassie Brilliant Blue GThe R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol.
How does Coomassie Brilliant Blue GWe found that the neutral ionic species of CBB binds to proteins by a combination of hydrophobic interactions and heteropolar bonding with basic amino acids.
What solvent is used in preparation of Coomassie brilliant blue?In classical protein staining protocols using Coomassie Brilliant Blue (CBB), solutions with high contents of toxic and flammable organic solvents (Methanol, Ethanol or 2-Propanol) and acetic acid are used for fixation, staining and destaining of proteins in a gel after SDS-PAGE.
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Coomassie brilliant blue r-250 staining solution sds
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